Chicken Interleukin 18,IL-18 ELISA KIT

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞白介素18(IL-18)酶聯免疫試劑盒（CSB-E10070Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL18含量。IL-18，又稱干擾素-γ誘導因子，是一種促炎細胞因子，屬于IL-1超家族。它主要由巨噬細胞和其他細胞產生，能夠誘導T細胞產生干擾素-γ。IL-18在免疫調節(jié)中起重要作用，參與先天性和適應性免疫反應的調節(jié)。其研究機制涉及IL-18與IL-18R的結合，以及通過激活下游信號通路來調節(jié)免疫細胞的功能。試劑盒檢測范圍為20 pg/mL-500 pg/mL，適用于家禽免疫學研究、疫苗效力評估或感染性疾病模型的建立。科研人員可通過精準分析雞類樣本中IL - 18的表達水平，探究其在傳染性法氏囊病、新城疫等病原體感染過程中的免疫調控機制，或評估營養(yǎng)干預、環(huán)境應激對家禽免疫功能的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
縮寫：

IL18
Uniprot No.：

Q8QFQ8
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue Homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-102
1:2	Average %	90
	Range %	82-99
1:4	Average %	93
	Range %	85-102
1:8	Average %	96
	Range %	92-100

回收率：

The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	81-99
EDTA plasma (n=4)	95	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
亞細胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

STRING: 9031.ENSGALP00000012772

UniGene: Gga.17593