Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit
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中文名稱：人Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E08082h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒（CSB-E08082h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養(yǎng)上清液、組織勻漿樣本中的Col Ⅰ含量。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于纖維化疾病模型研究、骨代謝機(jī)制探索以及再生醫(yī)學(xué)中膠原沉積的動(dòng)態(tài)分析。該產(chǎn)品兼容多種實(shí)驗(yàn)場景，包括體外細(xì)胞培養(yǎng)體系中膠原分泌的定量評(píng)估、動(dòng)物模型組織修復(fù)過程的生物標(biāo)志物追蹤，以及臨床前研究中Col Ⅰ代謝相關(guān)病理生理機(jī)制的解析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Collagen Type 1,Col 1,_x000D_Collagen Type I,Col I
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縮寫：Col Ⅰ
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種屬：Homo sapiens (Human)
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樣本類型：serum, urine, cell culture supernates, tissue homogenates
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檢測范圍：1.56 ng/mL-100 ng/mL
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靈敏度：0.39 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 80-92 1:2 Average % 100 Range % 91-110 1:4 Average % 105 Range % 92-110 1:8 Average % 93 Range % 86-100 -
回收率：
The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-99 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 100 2.921 2.985 2.953 2.767 50 2.356 2.378 2.367 2.181 25 1.826 1.875 1.851 1.665 12.5 1.221 1.256 1.239 1.053 6.25 0.796 0.800 0.798 0.612 3.12 0.512 0.546 0.529 0.343 1.56 0.302 0.326 0.314 0.128 0 0.188 0.184 0.186 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- A novel polypeptide inhibitor of MMP-1 attenuates the UVA-mediated skin aging PX Tang, JX Li, C Guo, Z Xia, XY Zheng, SY Zhu,Biochemical and Biophysical Research Communications,2025
- Lavender Exosome-Like nanoparticles attenuate UVB-Induced Photoaging via miR166-Mediated inflammation and collagen regulation S Li, F Liu, S Zhang, X Sun, X Li, Q Yue, L Su,Scientific Reports,2025
- Epac1 Inhibits Orbital Fibroblast Activation to Ameliorate Thyroid-Associated Orbitopathy-Like Features Through the JAK/STAT Signaling Pathway R Liu, X Xin, S Ma, J Guo, C Wu,Investigative Ophthalmology & Visual Science,2025
- The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease S Liu, J Yi, S Lu, J Cao, B Xie, Y Tan,Investigative Ophthalmology & Visual Science,2025
- The Reduction of Skin Photodamage by the Ectoine–Thermus thermophilus Cofermentation Products Y Wang, J Liang, P Zhang, G Yan,Journal of Cosmetic Dermatology,2025
- Lymphomonocytic Extracellular Vesicles Influence Fibroblast Proliferation and Collagen Production in Systemic Sclerosis G Argentino, B Olivieri, M Morandi, G Bonisoli,International Journal of Molecular Sciences,2025
- Tuberostemonine may alleviates proliferation of lung fibroblasts caused by pulmonary fibrosis A Tang, Y Liu, Q Ding, G Huang, Z Sha,International Journal of Immunopathology and Pharmacology,2024
- The Role of Collagen I, Magnesium and Phosphorus in Iraqi Meningioma Patients RZ Jasim,/,2024
- Skin antiaging effects of a multiple mechanisms hyaluronan complex F Chen,Skin Research and Technology,2023
- Secretome of human umbilical cord mesenchymal stem cell maintains skin homeostasis by regulating multiple skin physiological function X Wang,Cell and Tissue Research,2022
- Graphene Oxide Loaded on TiO2-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts X Cao,International journal of molecular sciences,2022
- Contribution of Adiponectin/Carnitine Palmityl Transferase 1A-Mediated Fatty Acid Metabolism during the Development of Idiopathic Pulmonary Fibrosis W Wu,Oxidative Medicine and Cellular Longevity,2022
- Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts Y Zhang,Food Science & Nutrition,2022
- Responses of human gingival fibroblasts to superhydrophilic hydrogenated titanium dioxide nanotubes C Wang,Colloids and Surfaces B: Biointerfaces,2020
- Biocompatibility and antibacterial properties of pure titanium surfaces coated with yttrium-doped hydroxyapatite Zhang K, et al,Journal of The Mechanical Behavior of Biomedical Materials,2019
- Chemical Fingerprint of Zn-Hydroxyapatite in the Early Stages of Osteogenic Differentiation Procopio A, et al,Pulmonary Pharmacology & Therapeutics,2019
- Berberine improves advanced glycation end products Zhang LN,Molecular Medicine Reports,2019
- Berberine improves advanced glycation end products-induced osteogenic differentiation responses in human periodontal ligament stem cells through the canonical Wnt/beta-catenin pathway Zhang LN,Molecular Medicine Reports,2019
- Growth of Endothelial Cells in Space and in Simulated Microgravity - a Comparison on the Secretory Level Kruger M, et al,Cellular Physiology and Biochemistry,2019
- Black Phosphorus Hydrogel Scaffolds Enhance Bone Regeneration via a Sustained Supply of Calcium-Free Phosphorus Huang K, et al,Acs Applied Materials & Interfaces,2018
- TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro Castiglioni S, et al,Scientific Reports,2018
- Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine Yang C.et al,Cell Physiol Biochem ,2017
- Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I. Frisch J.et al,Tissue Eng Part A. ,2015
- Interleukin-22 ameliorates liver fibrosis through miR-200a/beta-catenin Hu BL.et al,Scientific Reports,2016
- Enhanced osteoblast differentiation and osseointegration of a bio-inspired HA nanorod patterned pore-sealed MgO bilayer coating on magnesium Bo Li.et al,Materials Chemistry B,2016
- Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I Frisch J. et al,Tissue Eng Part A. ,2015
- Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015
- N-terminal 5-mer peptide analog P165 of amyloid precursor protein inhibits UVA-induced MMP-1 expression by suppressing the MAPK pathway in human dermal Wang Y et al,Eur J Pharmacol,2014
- Photoprotective effect of the N-terminal 5-mer peptide analog P165 of amyloid precursor protein in human dermal fibroblasts. Wang Y et al,Chin Med J (Engl),2014
產(chǎn)品評(píng)價(jià)
平均分:
樣品類型：血清 ,細(xì)胞培養(yǎng)上清液
樣品信息：人
稀釋比：其他 1:10
產(chǎn)品評(píng)價(jià)： human collagen type1 COL1 ELISA試劑盒檢測人血清/血漿，正常、模型和給藥組樣本數(shù)據(jù)很穩(wěn)定，復(fù)孔差異較小。血清/血漿結(jié)果相近，COL1靈敏度比COL3高，試劑盒穩(wěn)定方便。
By D老師
樣品類型：細(xì)胞培養(yǎng)上清液
樣品信息：人
稀釋比：其他 1:5
產(chǎn)品評(píng)價(jià)： 使用CSB-E08082h試劑盒檢測細(xì)胞中collagen 1含量，標(biāo)曲趨勢正確，數(shù)據(jù)完好。
By 張老師
樣品類型：細(xì)胞培養(yǎng)上清液
樣品信息：人
稀釋比：其他 1:20
產(chǎn)品評(píng)價(jià)： 我用CSB-E08082h產(chǎn)品檢測細(xì)胞上清呢膠原蛋白一型，標(biāo)曲正常。。。產(chǎn)品最后顯色時(shí)間延長到30min，OD范圍在0.1-0.3，COLⅠ試劑盒在實(shí)驗(yàn)中，樣本稀釋20-50倍。
By 李老師
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展：Collagen Ⅰ（Ⅰ型膠原）是一種主要存在于結(jié)締組織中的蛋白質(zhì)，最新研究表明它在疾病發(fā)生和治療中也起著重要作用。例如，肝纖維化和骨質(zhì)疏松等疾病都與膠原Ⅰ的異常表達(dá)有關(guān)。此外，一些藥物和生物制劑已經(jīng)用于調(diào)節(jié)膠原Ⅰ的合成和代謝，以治療相關(guān)疾病。
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