Human Interleukin 26,IL-26 ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
人白介素26(IL-26)酶聯(lián)免疫試劑盒（CSB-E11716h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織勻漿、細(xì)胞裂解物、組織培養(yǎng)上清液樣本中的IL26含量。IL26是一種白細(xì)胞介素，屬于IL-10家族，最初在皰疹病毒轉(zhuǎn)化的T細(xì)胞中高表達(dá)。它可能參與黏膜免疫的局部機(jī)制，具有促炎功能。IL26可激活STAT1、STAT3、MAPK1/3（ERK1/2）、JUN和AKT，誘導(dǎo)SOCS3、TNF-α和IL-8的表達(dá)，并促進(jìn)IL-8和IL-10的分泌及ICAM1的表面表達(dá)。IL26在多種炎癥性疾病中發(fā)揮作用，其具體作用機(jī)制尚在研究中。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品適用于基礎(chǔ)科研中IL - 26蛋白表達(dá)的動態(tài)監(jiān)測，包括但不限于炎癥性疾病模型構(gòu)建、病原體感染免疫應(yīng)答研究、腫瘤微環(huán)境分析等方向，為探索IL - 26在免疫調(diào)控、組織修復(fù)及疾病進(jìn)展中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
AK155 ELISA Kit; IL 26 ELISA Kit; IL-26 ELISA Kit; IL26 ELISA Kit; IL26_HUMAN ELISA Kit; Interleukin 26 ELISA Kit; Interleukin-26 ELISA Kit; Protein AK155 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, urine, tissue homogenates, cell lysates, cell culture supernates
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檢測范圍：
62.5 pg/mL-4000 pg/mL
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靈敏度：
15.6 pg/mL
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反應(yīng)時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Immunology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:4 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-104 |
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回收率：
| The recovery of human IL-26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
93-96 |
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| EDTA plasma (n=4) |
102 |
98-107 |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.749 |
2.798 |
2.774 |
2.616 |
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| 2000 |
2.315 |
2.364 |
2.340 |
2.182 |
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| 1000 |
1.843 |
1.889 |
1.866 |
1.708 |
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| 500 |
1.399 |
1.381 |
1.390 |
1.232 |
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| 250 |
0.899 |
0.888 |
0.894 |
0.736 |
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| 125 |
0.483 |
0.487 |
0.485 |
0.327 |
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| 62.5 |
0.316 |
0.329 |
0.323 |
0.165 |
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| 0 |
0.157 |
0.159 |
0.158 |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-26 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IL-26 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Squirt bottle, manifold dispenser, or automated microplate washer.
- Absorbent paper for blotting the microtiter plate.
- 100ml and 500ml graduated cylinders.
- Deionized or distilled water.
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
- Timer
- Test tubes for dilution.
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻(xiàn)
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Increased IL-26 associates with markers of hyperinflammation and tissue damage in patients with acute COVID-19
EI Cardenas, S Ekstedt, K Piersiala, M Petro, A Karlsson, ? K?gedal, S Kumlien Georén,Frontiers in Immunology,2022
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Systemic increase in IL-26 is associated with severe COVID-19 and comorbid obstructive lung disease
EI Cardenas, J Robertson, S Misaghian,Frontiers in Immunology,2024
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Human neutrophils drive skin autoinflammation by releasing interleukin (IL)-26
A Baldo,The Journal of experimental medicine,2024
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Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen
MA Kovach,/,2023
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Differentiation of IL-26+ TH17 intermediates into IL-17A producers via epithelial crosstalk in psoriasis
A Fries,Nature communications,2023
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Involvement of IL-26 in bronchiolitis obliterans syndrome but not in acute rejection after lung transplantation
JM Magnusson,Respiratory research,2022
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Single-cell atlas of colonic CD8+ T cells in ulcerative colitis
D Corridoni,Nature Medicine,2020
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IL-1β Induces the Rapid Secretion of the Antimicrobial Protein IL-26 from Th17 Cells
Weiss DI, et al,Journal of Immunology,2019
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The cytokine interleukin-26 as a biomarker in pediatric asthma
Konradsen JR.et al,Respir Res.,2016
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Regulation of Pulmonary Graft-versus-Host Disease by IL-26+ CD26+ CD4 T Lymphocytes
Ohnuma K et al,J Immunol,2015
靶點(diǎn)詳情
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功能：
May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin.
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基因功能參考文獻(xiàn)：
- this paper shows that IL-26 is overexpressed in Behcet's disease and enhances Th17 related -cytokines PMID: 28811236
- The varIL26 genotype is associated with reduced PMN capacity to kill bacteria. A varIL26 genotype is associated with decreased levels of anti-TNF-alpha in CD patients. IL26 may help explain the role of bactDNA as a risk factor of flare in CD patients. PMID: 28879509
- IL-26 activates STAT1/3 and leads to the induction of IL-6 and IL-8 expression in non-transformed cells derived from human colon. PMID: 28852311
- IL-26 levels are higher in synovial fluid compared to plasma in spondyloarthritis. IL-26 was identified in axial facet joints of spondyloarthritis patients. Myofibroblasts from the spondyloarthritis synovium produce large amounts of IL-26. IL-26 induces bone mineralization in human osteoblasts. PMID: 28365787
- this study shows that IL-26 is a unique cationic protein more similar to a soluble pattern recognition receptor than to conventional cytokines PMID: 28356384
- this study shows that single nucleotide polymorphism near IL-26 gene is associated with familial vitiligo and existence of halo nevi in Estonian patients PMID: 26429320
- Our study indicates that IL-26 is a potential biomarker of disease severity in pediatric asthma without signs of Th2-mediated inflammation. PMID: 27029915
- IL-26 is emerging as a potentially important player in host defense and may also be a pathogenic factor in the chronic inflammatory disorders of humans. [Review] PMID: 26202572
- The negative influence of IL-26 on the anti-mycobacterial activity and its constitutive presence in both serum and monocyte supernatants prompt a proposal that IL26 as a candidate gene for tuberculosis susceptibility. PMID: 25157980
- IL-26 differentially modulates the infection by different enveloped viruses. PMID: 23875025
- Elevated levels of IL-26 in human gastric cancer promote proliferation and survival by modulating STAT1/STAT3 signaling. PMID: 23704922
- IL-26 appears as a novel proinflammatory cytokine, located upstream of the proinflammatory cascade, that may constitute a promising target to treat rheumatoid arthritis and chronic inflammatory disorders. PMID: 23055831
- Identify a second enhancer element positioned between IL26 and IFNG required for both IL26 and IFNG expression. One function of this enhancer is to facilitate recruitment of RNA polymerase II to promoters of both genes. PMID: 22622197
- common polymorphisms in the IFNgamma/IL-26 gene region may contribute to sex bias in susceptibility to rheumatoid arthritis, by distorting the propensity of female carriers versus male carriers to contract this disease. PMID: 14558082
- The active receptor complex for IL-26 is a heterodimer composed of two receptor proteins: IL-20 receptor 1 and IL-10 receptor 2. Signaling through the IL-26 receptor results in activation of STAT1 and STAT3. PMID: 14764663
- sensitivity to recombinant interleukin-26(IL-26) of various cell lines strictly correlated with the expression of IL-20 receptor 1 and blocking antibodies against either IL-10 receptor 2 or IL-20 receptor 1 inhibited IL-26-dependent signal transduction PMID: 15178681
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亞細(xì)胞定位：
Secreted.
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蛋白家族：
IL-10 family
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組織特異性：
Expressed in HVS transformed T-cells but not other T-cell lines or primary stimulated T-cells. Expressed in colonic T-cells including Th17 inflammatory T-cells; the expression is significantly increased in serum of patients with Crohn's disease (at protei
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