Mouse Fetuin A ELISA Kit
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中文名稱：小鼠胎球蛋白A酶聯(lián)免疫試劑盒
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貨號：CSB-E14042m
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規(guī)格：96T/48T
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價格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠胎球蛋白A酶聯(lián)免疫試劑盒（CSB-E14042m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AHSG含量。AHSG即α2-HS糖蛋白，是肝臟合成的血漿糖蛋白。研究顯示它參與多種生理病理過程，如調(diào)節(jié)細(xì)胞生長、免疫反應(yīng)及礦物質(zhì)代謝等。其機(jī)制可能包括與細(xì)胞表面受體結(jié)合，影響信號通路傳導(dǎo)，進(jìn)而調(diào)節(jié)細(xì)胞的功能與行為。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，靈敏度為1.56 ng/mL。適用于科研領(lǐng)域探究小鼠模型中肝臟功能異常、糖脂代謝紊亂、心血管疾病或骨代謝相關(guān)機(jī)制。其寬泛的樣本兼容性可支持血清/血漿樣本的體液分析，或通過組織勻漿檢測特定器官的蛋白表達(dá)差異，為構(gòu)建疾病模型、評估干預(yù)效果提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Ahsg ELISA kit; FetuaAlpha-2-HS-glycoprotein ELISA kit; Countertrypin ELISA kit; Fetuin-A ELISA kit
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縮寫：AHSG
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：6.25 ng/mL-400 ng/mL
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靈敏度：1.56 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Metabolism
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(ng/ml)
52.627
50.900
51.353
52.901
49.725
50.990
SD
0.041
0.035
0.037
0.054
0.049
0.047
CV(%)
5.216
4.563
4.793
6.844
6.499
6.120
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:10
Average %
95
Range %
87-103
1:20
Average %
95
Range %
84-105
1:40
Average %
90
Range %
83-103
1:80
Average %
93
Range %
82-107
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回收率：
The recovery of mouse fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
90-98
Heparin plasma (n=4)
91
85-96
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
400
2.657
2.704
2.681
2.547
200
1.905
2.002
1.954
1.820
100
1.259
1.279
1.269
1.135
50
0.714
0.758
0.736
0.602
25
0.426
0.442
0.434
0.300
12.5
0.361
0.348
0.355
0.221
6.25
0.257
0.244
0.251
0.117
0
0.134
0.133
0.134
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數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Probably involved in differentiation.
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基因功能參考文獻(xiàn)：
- Downregulation of adiponectin in inflamed adipocyte by fetuin-A through the mediation of Wnt3a and PPARgamma is a new report. PMID: 27720679
- FETUA expression and ER stress markers in the liver and serum of HFD mice were decreased by melatonin treatment. In conclusion, melatonin can improve hepatic insulin resistance and hepatic steatosis through reduction in ER stress and the resultant AHSG expression. PMID: 29607540
- Impairment of energy sensors, SIRT1 and AMPK, in lipid induced inflamed adipocyte is regulated by Fetuin A. PMID: 29030114
- The growth plate slippage occurred in 70% of Ahsg-/- mice of both sexes around three weeks postnatal. PMID: 29088242
- AHSG is produced in bone, mainly in osteocytes and also show for the first time that its production is modulated by FGF23. PMID: 26476373
- These findings establish urinary Fetuin-A as a sensitive biomarker of the progression of autosomal dominant polycystic kidney disease. PMID: 25888842
- Plasma levels of the calcification inhibitor fetuin-A were significantly decreased in CKD animals compared to controls. PMID: 24994603
- fetuin-A regulates endochondral ossification through mineralization inhibition and lipid (hormone) binding [review] PMID: 23277412
- Our results show that CSF fetuin-A is a biomarker of disease activity and natalizumab response in multiple sclerosis PMID: 23439582
- lipid-induced FetA from adipocytes is an efficient chemokine for macrophage migration and polarization PMID: 23943623
- FetA, via its terminal galactoside moiety, directly binds Leu100-Gly123 & Thr493-Thr516 in Tlr4, fulfilling the requirement of an endogenous ligand for TLR4 through which lipids induce insulin resistance. PMID: 22842477
- Our results show that fetuin-A has a developmental rather than a structural role in murine bone. PMID: 23091616
- Fetuin-A-containing calciprotein particles facilitate the clearance of mineral debris by macrophages via SR-A. PMID: 22753077
- ER stress induced by high glucose and palmitate increases the expression of fetuin-A and further contributes to the development of insulin resistance PMID: 22619360
- fetuin-A is protective against lethal systemic inflammation partly by inhibiting active HMGB1 release PMID: 21347455
- Fetuin-A deficiency is associated with soft tissue calcification in mice and humans. PMID: 21659653
- breast cancer but also of other tumors whose initiation and promotion are under the tight control of TGF-beta. PMID: 20847285
- Fetuin-A expressions were negatively correlated with the liver pathological scores and TNFa levels in fulminant hepatic failure. PMID: 18578995
- alpha 2-HS glycoprotein/fetuin, a transforming growth factor-beta/bone morphogenetic protein antagonist, regulates postnatal bone growth and remodeling PMID: 11901155
- Improved insulin sensitivity and resistance to weight gain in knockout mice PMID: 12145157
- role as a systemically acting inhibitor of ectopic calcification PMID: 12897203
- Regulation of BMP-induced ectopic bone formation by Ahsg. PMID: 15885488
- Onset of malaria infection is delayed in fetuin-A-deficient mice compared to that in wild-type C57BL/6 mice challenged with Plasmodium berghei sporozoites PMID: 16113307
- Fetuin-A knockout (fetuin-KO) mice spontaneously develop widespread soft tissue calcification, including significant myocardial calcification, whereas larger arteries are spared. PMID: 16177000
- The absence of fetuin may contribute to the improvement of insulin sensitivity associated with aging. PMID: 17011519
- Fetuin-A deficiency, chronic kidney disease, and high phosphate diet act synergistically in the pathogenesis of extraosseous calcification. PMID: 17389622
- fetuin recognized by anti-LY-R antibodies is an immunogenic form associated only with the tumorigenic LY-R cells and might be involved in tumor progression in this lymphoma. PMID: 19194829
- inhibits pathologic calcification in both the soft tissue and vasculature, even in the setting of atherosclerosis PMID: 19389852
- inhibition of ectopic calcification PMID: 12556469
- inhibition of ectopic calcification PMID: 9395485
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亞細(xì)胞定位：Secreted.
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蛋白家族：Fetuin family
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組織特異性：Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development.
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數(shù)據(jù)庫鏈接：
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