Rat Acylated Ghrelin (AG) ELISA Kit
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中文名稱：大鼠酰基化饑餓素(AG)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E13167r
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規(guī)格：96T/48T
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促銷：
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠酰基化饑餓素(AG)酶聯(lián)免疫試劑盒（CSB-E13167r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的AG含量。大鼠酰基化饑餓素（AG）是一種由胃內(nèi)分泌細(xì)胞產(chǎn)生的激素，具有促進(jìn)食欲和調(diào)節(jié)能量平衡的作用。其活性形式是通過(guò)辛酰化修飾的，能夠激活GHSR受體，影響生長(zhǎng)激素釋放、食欲和能量代謝。研究顯示，AG在調(diào)節(jié)血糖和胰島素抵抗中扮演重要角色，其水平變化與肥胖和糖尿病等代謝性疾病密切相關(guān)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品適用于動(dòng)物模型研究，如肥胖、糖尿病或攝食調(diào)控機(jī)制的探索，也可用于體外藥物篩選或激素分泌相關(guān)的基礎(chǔ)研究，為科研人員提供穩(wěn)定可靠的數(shù)據(jù)支持，滿足代謝調(diào)控、內(nèi)分泌系統(tǒng)研究等領(lǐng)域的實(shí)驗(yàn)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：AG
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：6.25 pg/mL-400 pg/mL
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靈敏度：1.56 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Others
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat AG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 87-101 1:2 Average % 86 Range % 80-92 1:4 Average % 93 Range % 90-95 1:8 Average % 95 Range % 92-98 -
回收率：
The recovery of rat AG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 95-104 EDTA plasma (n=4) 97 95-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 400 2.690 2.699 2.695 2.502 200 1.885 1.899 1.892 1.699 100 1.162 1.198 1.180 0.987 50 0.798 0.760 0.779 0.586 25 0.581 0.535 0.558 0.365 12.5 0.388 0.376 0.382 0.189 6.25 0.301 0.321 0.311 0.118 0 0.191 0.195 0.193 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Changes in ghrelin, CCK, GLP-1, and peroxisome proliferator-activated receptors in a hypoxia-induced anorexia rat model Duraisamy AJ. et al,Endokrynol Pol,2015
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