Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit
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中文名稱(chēng)：大鼠粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E04570r
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)酶聯(lián)免疫試劑盒（CSB-E04570r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CSF2含量。CSF2，即粒細(xì)胞-巨噬細(xì)胞集落刺激因子（GM-CSF），是一種由多種細(xì)胞分泌的細(xì)胞因子，最初被定義為一種造血因子。它通過(guò)調(diào)節(jié)骨髓細(xì)胞生成和免疫反應(yīng)，在免疫調(diào)節(jié)、炎癥反應(yīng)和腫瘤進(jìn)展中扮演關(guān)鍵角色。CSF2通過(guò)激活信號(hào)通路，如JAK-STAT和PI3K-Akt，影響細(xì)胞的增殖、分化和功能，并在多種疾病的治療中具有潛在應(yīng)用價(jià)值。試劑盒檢測(cè)范圍為15/6 pg/ml- 1000 pg/ml，靈敏度為3.9 pg/ml。適用于科研領(lǐng)域中對(duì)大鼠感染性疾病模型、免疫調(diào)控機(jī)制、造血功能研究以及藥物干預(yù)效果的評(píng)價(jià)。實(shí)驗(yàn)操作兼容多種生物樣本類(lèi)型，可滿(mǎn)足體外實(shí)驗(yàn)中GM - CSF的動(dòng)態(tài)監(jiān)測(cè)需求，為探索免疫相關(guān)疾病的分子機(jī)制或評(píng)估實(shí)驗(yàn)處理對(duì)細(xì)胞因子表達(dá)的影響提供可靠工具，廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)、藥理學(xué)及免疫學(xué)等研究場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
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縮寫(xiě)：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類(lèi)型：serum, plasma, tissue homogenates
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檢測(cè)范圍：15.6 pg/ml - 1000 pg/ml
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靈敏度：3.9 pg/ml
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Immunology
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線(xiàn)性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
85
Range %
81-89
1:2
Average %
97
Range %
92-101
1:4
Average %
91
Range %
86-95
1:8
Average %
103
Range %
99-107
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回收率：
The recovery of rat GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
88
84-92
EDTA plasma (n=4)
94
90-98
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標(biāo)準(zhǔn)曲線(xiàn)：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.468
2.326
2.397
2.210
500
1.857
1.792
1.825
1.638
250
1.213
1.250
1.232
1.045
125
0.785
0.813
0.799
0.612
62.5
0.492
0.468
0.480
0.293
31.2
0.331
0.347
0.339
0.152
15.6
0.289
0.271
0.280
0.093
0
0.184
0.189
0.187
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數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
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基因功能參考文獻(xiàn)：
- Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID: 28550897
- Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID: 27364613
- The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID: 25677907
- Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID: 12071442
- GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID: 14557677
- We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID: 14651956
- GM-CSF may play a significant role in follicular development PMID: 15958400
- GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID: 16276414
- GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID: 16877635
- GM-CSF acts as a neuroprotective protein in the CNS PMID: 17457367
- Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID: 17478029
- GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID: 17953750
- G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID: 18832728
- IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID: 19022682
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亞細(xì)胞定位：Secreted.
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蛋白家族：GM-CSF family
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數(shù)據(jù)庫(kù)鏈接：
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