Rat Interleukin 3,IL-3 ELISA KIT

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Rat Interleukin 3,IL-3 ELISA KIT

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說明書下載

中文名稱：

大鼠白介素3(IL-3)酶聯免疫試劑盒
貨號：

CSB-E07436r
規格：

96T/48T
價格：

￥3200/￥2500
其他：

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產品描述：

大鼠白介素3(IL-3)酶聯免疫試劑盒（CSB-E07436r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL3含量。IL3，即白細胞介素-3，是一種由激活的T細胞和其他細胞產生的細胞因子，主要在造血過程中發揮作用，調控粒細胞和單核細胞-巨噬細胞的產生、分化和功能。其研究機制涉及IL3如何通過激活細胞內信號通路，如Ras-Raf-ERK和JAK/STAT，來促進造血細胞的生長和分化。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于科研領域中對IL-3表達水平的研究，例如在造血系統疾病模型、感染性疾病動物實驗或免疫相關病理機制探討中，通過檢測血液或組織樣本中的IL-3濃度變化，揭示其在炎癥反應、細胞分化及免疫調控中的作用規律。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Il3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
縮寫：

IL3
Uniprot No.：

P04823
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	100
	Range %	95-113
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	92-104

回收率：

The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.436	2.276	2.356	2.214
100	1.975	2.014	1.995	1.853
50	1.435	1.372	1.404	1.262
25	0.868	0.913	0.891	0.749
12.5	0.553	0.519	0.536	0.394
6.25	0.387	0.407	0.397	0.255
3.12	0.285	0.276	0.281	0.139
0	0.144	0.140	0.142

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
亞細胞定位：

Secreted.
蛋白家族：

IL-3 family
組織特異性：

Activated T-cells, mast cells, natural killer cells.
數據庫鏈接：

UniGene: Rn.10652

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.