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Rat leukotriene D4(LTD4) ELISA kit

文獻引用 (2) 說明書下載

產品詳情

產品描述：

大鼠白三烯D4(LTD4)酶聯免疫試劑盒（CSB-EQ027312RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液樣本中的LTD4含量。LTD4即白三烯D4，是一種重要的生物活性介質。它參與多種炎癥和過敏反應，與哮喘、過敏性鼻炎等疾病密切相關。研究機制主要聚焦于其與受體結合后觸發細胞內信號傳導，導致氣道收縮、炎癥細胞浸潤等一系列病理生理變化。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，本產品適用于基礎科研中LTD4相關通路研究，如呼吸道疾病動物模型評估、炎癥信號機制探索或抗炎藥物篩選等實驗場景，為體外實驗提供穩定可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

LTD4
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LTD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	103
	Range %	99-108
1:4	Average %	91
	Range %	85-97
1:8	Average %	94
	Range %	91-98

回收率：

The recovery of rat LTD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	92	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.