ABI2 Recombinant Monoclonal Antibody
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中文名稱：ABI2 Recombinant Monoclonal Antibody
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貨號：CSB-RA582667A0HU
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規格：￥1320
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圖片：
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Immunofluorescence staining of MCF-7 cell with CSB-RA582667A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA582667A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Abelson interactor 2 antibody; ABI 2 antibody; Abi-2 antibody; ABI2 antibody; ABI2_HUMAN antibody; ABI2B antibody; Abl binding protein 3 antibody; Abl interacting protein 1 (SH3 containing protein) antibody; Abl interactor 2 antibody; Abl interactor protein 2b antibody; Abl-binding protein 3 antibody; AblBP3 antibody; AIP 1 antibody; Arg binding protein 1 antibody; Arg protein tyrosine kinase binding protein antibody; Arg-binding protein 1 antibody; ArgBP1 antibody; ARGBPIA antibody; ArgBPIB antibody; OTTHUMP00000163783 antibody; OTTHUMP00000163784 antibody; OTTHUMP00000206182 antibody; OTTHUMP00000206183 antibody; OTTHUMP00000206184 antibody; OTTHUMP00000206185 antibody; SSH3BP2 antibody
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反應種屬：Human
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免疫原：A synthesized peptide from human ABI2 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：12F4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, IF, FC
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推薦稀釋比：
Application Recommended Dilution IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation. Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge. Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain. In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth. Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface. Also required for nascent adherens junction assembly in epithelial cells.
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基因功能參考文獻：
- TIS21 attenuated Doxorubicin-induced cancer cell senescence by inhibiting linear actin nucleation via Nox4-ROS-ABI2-DRF signal cascade PMID: 27932314
- Abl-interactor 2 (ABI2) is a novel MLL translocation partner in acute myeloid leukemia. PMID: 22304832
- Abi-2 may have a key regulatory function in leiomyomas cellular/tissue structural organization during growth and regression PMID: 16488906
- disruption of the interaction between Bcr-Abl and Abi1 by mutations either in Bcr-Abl or Abi1 not only abolished tyrosine phosphorylation of Abi1 and membrane translocation of Abi1/WAVE2 PMID: 17389688
- TRIM32 is a novel oncogene that promotes tumor growth, metastasis, and resistance to anticancer drugs via degradation of Abl-interactor 2 PMID: 18632609
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亞細胞定位：Cytoplasm. Nucleus.; [Isoform 1]: Cell projection, lamellipodium. Cell projection, filopodium. Cytoplasm, cytoskeleton. Cell junction, adherens junction.
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蛋白家族：ABI family
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組織特異性：Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.
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數據庫鏈接：
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