MRPS15 Recombinant Monoclonal Antibody
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								中文名稱:MRPS15 Recombinant Monoclonal Antibody
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								貨號:CSB-RA644699A0HU
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								規格:¥1320
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								圖片:- 
														
														IHC image of CSB-RA644699A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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														Immunofluorescence staining of HepG2 cell with CSB-RA644699A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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														Overlay Peak curve showing Hela cells stained with CSB-RA644699A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
 
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								其他:
產品詳情
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											Uniprot No.:
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											基因名:MRPS15
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											別名:28S ribosomal protein S15 antibody; 28S ribosomal protein S15, mitochondrial [Precursor] antibody; DC37 antibody; FLJ11564 antibody; mitochondrial antibody; mitochondrial ribosomal protein S15 antibody; MPR S15 antibody; MRP-S15 antibody; MRPS15 antibody; RPMS15 antibody; RT15_HUMAN antibody; S15mt antibody
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											反應種屬:Human
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											免疫原:A synthesized peptide from human MRPS15 protein
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											免疫原種屬:Homo sapiens (Human)
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											標記方式:Non-conjugated
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											克隆類型:Monoclonal
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											抗體亞型:Rabbit IgG
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											純化方式:Affinity-chromatography
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											克隆號:9B11
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											濃度:It differs from different batches. Please contact us to confirm it.
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											保存緩沖液:Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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											產品提供形式:Liquid
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											應用范圍:ELISA, IHC, IF, FC
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											推薦稀釋比:Application Recommended Dilution IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 
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											Protocols:
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											儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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											貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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											用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關產品
靶點詳情
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											亞細胞定位:Mitochondrion.
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											蛋白家族:Universal ribosomal protein uS15 family
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											數據庫鏈接:
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