TXNDC12 Recombinant Monoclonal Antibody
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中文名稱：TXNDC12 Recombinant Monoclonal Antibody
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貨號：CSB-RA995859A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HepG2 whole cell lysate(30μg), HT-29 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), U-251MG whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), K562 whole cell lysate(30μg), Rat liver tissue lysate(30μg)
All lanes:TXNDC12 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time：3min -
IHC image of CSB-RA995859A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing HepG2 cells stained with CSB-RA995859A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：TXNDC12
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別名：AG1 antibody; AGR1 antibody; anterior gradient homolog 1 antibody; endoplasmic reticulum protein ERp19 antibody; Endoplasmic reticulum resident protein 18 antibody; Endoplasmic reticulum resident protein 19 antibody; endoplasmic reticulum thioredoxin superfamily member, 18 kDa antibody; ER protein 18 antibody; ER protein 19 antibody; ERP 18 antibody; ERP16 antibody; ERp18 antibody; ERp19 antibody; hAG 1 antibody; hAG1 antibody; hTLP19 antibody; PDIA16 antibody; protein disulfide isomerase family A, member 16 antibody; thioredoxin domain containing 12 (endoplasmic reticulum antibody; Thioredoxin domain-containing protein 12 antibody; thioredoxin like protein p19 antibody; Thioredoxin-like protein p19 antibody; TLP19 antibody; TXD12_HUMAN antibody; TXNDC12 antibody
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反應種屬：Human, Rat
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免疫原：A synthesized peptide from human TXNDC12 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：21A7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Possesses significant protein thiol-disulfide oxidase activity.
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基因功能參考文獻：
- We conclude that ERp19 contributes to tumorigenicity and metastasis of gastric cancer PMID: 25940440
- ERp18 shows specificity towards a component of the complement cascade, pentraxin-related protein PTX3. PMID: 19887585
- putative physiological role for endoplasmic reticulum thioredoxin superfamily member p18(ERp18) in native disulfide bond formation is discussed PMID: 12761212
- ERp16 mediates disulfide bond formation in the ER and plays an important role in cellular defense against prolonged ER stress PMID: 18628206
- the solution structure of oxidized ERp18 as determined using NMR spectroscopy PMID: 19361226
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亞細胞定位：Endoplasmic reticulum lumen.
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組織特異性：Widely expressed.
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數(shù)據(jù)庫鏈接：
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