Histone H2A type 1-B/E Recombinant Monoclonal Antibody
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中文名稱(chēng)：組蛋白 H2A type 1-B/E重組抗體
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貨號(hào)：CSB-RA010385A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in HepG2 whole cell lysate
All lanes Histone H2A type 1-B/E antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 KDa
Observed band size: 13 KDa -
Immunofluorescence staining of Hela cells with CSB-RA010385A0HU at 1:168,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA010385A0HU是一種特異性識(shí)別組蛋白H2A亞型的高純度抗體，靶向高度保守的組蛋白H2A家族成員。該抗體通過(guò)重組技術(shù)制備，具有批次間一致性?xún)?yōu)勢(shì)，適用于多種實(shí)驗(yàn)場(chǎng)景。經(jīng)嚴(yán)格驗(yàn)證，其在Western Blot中可特異性識(shí)別目標(biāo)蛋白，推薦稀釋比例為1:500-1:5000；在免疫熒光（IF）實(shí)驗(yàn)中能清晰定位細(xì)胞核內(nèi)靶標(biāo)，推薦稀釋比例為1:30-1:200，同時(shí)適用于ELISA檢測(cè)。作為核小體核心成分，H2A在染色質(zhì)組裝、DNA損傷修復(fù)及表觀遺傳調(diào)控中發(fā)揮關(guān)鍵作用，本產(chǎn)品可支持染色質(zhì)結(jié)構(gòu)分析、基因表達(dá)調(diào)控機(jī)制研究以及細(xì)胞周期相關(guān)實(shí)驗(yàn)。其高靈敏度和低交叉反應(yīng)性特點(diǎn)使其成為表觀遺傳學(xué)、分子生物學(xué)及細(xì)胞生物學(xué)領(lǐng)域基礎(chǔ)研究的可靠工具。
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Uniprot No.：
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基因名：HIST1H2AB
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別名：H2a 615 antibody; H2A antibody; H2A GL101 antibody; H2A histone family member A antibody; H2A.1 antibody; H2A.2 antibody; H2A/a antibody; H2A/m antibody; H2A/O antibody; H2A/q antibody; H2A1B_HUMAN antibody; H2AFA antibody; H2AFE antibody; H2AFL antibody; H2AFM antibody; H2AFO antibody; H2AFQ antibody; HIST1H2AE antibody; HIST1H2AJ antibody; HIST2H2AA antibody; HIST2H2AA3 antibody; HIST2H2AB antibody; HIST2H2AC antibody; Histone 1 H2ae antibody; Histone 2 H2aa3 antibody; Histone 2 H2ab antibody; Histone 2 H2ac antibody; Histone H2A type 1 B antibody; Histone H2A type 1 C antibody; Histone H2A type 1 E antibody; Histone H2A type 1 J antibody; Histone H2A type 1-B/E antibody; Histone H2A.2 antibody; Histone H2A/a antibody; Histone H2A/m antibody; MGC74460 antibody
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類(lèi)型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：1H12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:30-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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亞細(xì)胞定位：Nucleus. Chromosome.
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蛋白家族：Histone H2A family
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數(shù)據(jù)庫(kù)鏈接：
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