Recombinant Human Cyclin-T1 (CCNT1)

品名簡稱：

Recombinant Human CCNT1 protein
貨號：

CSB-EP004832HU
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

CCNT1
Uniprot No.：

O60563
別名：

(CycT1)(Cyclin-T)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

86.7 kDa
表達區域：

1-726aa
氨基酸序列

MEGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVTKSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSRSGNTDKPRPPPLPSEPPPPLPPLPK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).; (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
基因功能參考文獻：
1. ASF/SF2 is identified as a splicing regulator of cyclin T1, which contributes to the control of the subsequent transcription events. PMID: 28422315
2. The TAR central loop contacts the CycT1 Tat-TAR recognition motif (TRM) and the second Tat Zn(2+)-binding loop. Hydrogen-deuterium exchange (HDX) shows that AFF4 helix 2 is stabilized in the TAR complex despite not touching the RNA, explaining how it enhances TAR binding to the SEC 50-fold. PMID: 27731797
3. Quantitative measurement of the molecular interactions among Tat, CycT1 and CDK9 has showed that any third molecule enhances the binding between the other two molecules. These findings suggest that each component of the Tat:P-TEFb complex stabilizes the overall complex, thereby supporting the efficient transcriptional elongation during viral RNA synthesis. PMID: 27193293
4. the introduction of HIV-1 Tat and Mg12+ ion resulted in the active site architectural characteristics of phosphorylated CDK9/cyclin T1 complex PMID: 25909811
5. AFF4 is positioned to make unexpected direct contacts with HIV Tat, and Tat enhances P-TEFb/CCNT1 affinity for AFF4. PMID: 23471103
6. Plk1 negatively regulates the RNA polymerase II-dependent transcription through inhibiting the activity of cyclin T1/Cdk9 complex. PMID: 23977272
7. The sequence domain of human cytomegalovirus pUL97 responsible for the interaction with cyclin T1 was between amino acids 231-280. PMID: 24351800
8. the release of P-TEFb from the 7SK snRNP led to increased synthesis of HEXIM1 but not HEXIM2 PMID: 24515107
9. All of the hCD4/R5/cT1 mice developed disseminated infection of tissues that included the spleen, small intestine, lymph nodes and lungs after intravenous injection with an HIV-1 infectious molecular clone PMID: 23691059
10. positive transcription elongation factor b (P-TEFb, made of CDK9 and CycT1) is released from its inhibitory complex by histone deacetylase inhibitors, which also activate HIV transcription PMID: 23539624
11. CYCT1b is a newly identified cyclin T1 splice variant whose expression leads to direct inhibition of TAT-transactivated transcription of the HIV-1 LTR promoter PMID: 23569210
12. Structural determination of a short-lived conformational state between CCNT1 and CDK9 that is adopted during the phosphorylation cycle. PMID: 22959624
13. The authors find that resting CD4(+) T cells, whether naive or memory and independent of their HIV infection status, have low levels of Cyclin T1 and T-loop-phosphorylated CDK9, which increase upon activation. PMID: 23152527
14. N-terminal residues in cyclin T1 are specifically involved in the binding of cyclin T1 to HEXIM1 but not to Tat. PMID: 22342181
15. MiR-27b, miR-29b, miR-223, and miR-150 are regulators of cyclin T1 protein levels and HIV-1 replication in resting CD4+ T cells. PMID: 22205749
16. The role of the CycT1 N-terminal region in Tat action, was investigated. PMID: 21763494
17. This investigation reveals a novel mechanism of cyclin T1 regulation and establishes NF90 as a regulator of HIV-1 replication during both productive infection and induction from latency. PMID: 21763496
18. Cyclin K inhibits HIV-1 gene expression and replication by interfering with cyclin-dependent kinase 9 (CDK9)-cyclin T1 interaction in Nef-dependent manner. PMID: 21555514
19. HCMV inter-regulation with CDK9/cyclin T1 is at least partly based on a pUL69-cylin T1 interaction. PMID: 21450947
20. study showed that P-TEFb and Dengue virus (DENV) core protein work in concert to enhance IL-8 gene expression by DENV infection PMID: 20618343
21. results suggest that cyclin T1/CDK9 serves as an adapter to mediate the interaction of influenza A virus RNA-dependent RNA polymerase and RNA Pol II and promote viral transcription PMID: 20943989
22. Cyclin T1 oncogenic function and suggest a role for this protein in controlling cell cycle probably via Rb/E2F1 pathway. PMID: 20714219
23. binding to Cyclin T1 induces an asymmetry or sterical hindrance in the first coiled coil segment of dimeric Hexim1 that disallows formation of a 2:2 complex. PMID: 20210365
24. Activity inhibited by 7SK small nuclear RNA. PMID: 11713533
25. P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. PMID: 11884399
26. CDK9 has the intrinsic property to shuttle between nucleus and cytoplasm, and enhanced expression of cyclin T1 promotes its nuclear localization. PMID: 12115727
27. P-TEFb is a key mediator of Myc activated transcription by stimulating elongation PMID: 12177005
28. fluctuation in CycT1 levels during human macrophage differentiation may be involved in the regulation of HIV-1 replication PMID: 12368300
29. chimeras between kinase-inactive mutant Cdk9 and truncated cyclin T1 proteins efficiently inhibit Tat transactivation and human immunodeficiency virus gene expression PMID: 12368330
30. Tat and trans-activation-responsive (TAR) RNA-independent induction of HIV-1 long terminal repeat by this protein requires Sp1 PMID: 12458222
31. Cyclin T1 binding to HIV-1 tat is regulated by the differential acetylation of tat PMID: 12486002
32. cyclin T1 binds with granulin to inhibit transcription PMID: 12588988
33. the transcriptional repressor PIE-1 from Caenorhabditis elegans binds Cyclin T1 via an alanine-containing heptapeptide repeat and inhibits transcriptional elongation {cyclin T1) PMID: 12651893
34. recruitment to nuclear bodies via direct interaction with PML protein PMID: 12727882
35. Since Tat also binds to this cyclin T1 domain and the association between 7SK RNA/MAQ1 and P-TEFb competes with the binding of Tat to cyclin T1, we speculate that the TAR RNA/Tat lentivirus system has evolved to subvert the cellular 7SK RNA/MAQ1 system PMID: 12832472
36. major portion of nuclear P-TEFb is sequestered and inactivated by the coordinated actions of the 7SK snRNA PMID: 14627702
37. RNAi-mediated gene silencing of P-TEFb in HeLa cells was not lethal and inhibited Tat transactivation and HIV-1 replication in host cells PMID: 14963154
38. review of work indicating under some circumstances TAK/P-TEFb is likely to be limiting for HIV replication in CD4+ T cells and macrophages; review of mechanisms of regulation of the TAK/P-TEFb subunits in these cell types PMID: 15049426
39. NUFIP and P-TEFb with BRCA1 activate transcription by RNA polymerase II PMID: 15107825
40. a histidine-rich stretch in CycT1 was found to direct the transcriptional activity of this P-TEFb complex when tethered artificially to DNA. PMID: 15563843
41. HEXIM1 and HEXIM2 maintain the balance between active and inactive forms of P-TEFb, a heterodimeric complex composed of cyclin-dependent kinase 9 and cyclin T. PMID: 15713661
42. downregulation of hCycT1 did not cause apoptotic cell death PMID: 15913611
43. increased estrogen down regulated gene 1 expression results in inhibition of cyclin T1 recruitment and estrogen receptor 1 DNA binding PMID: 15940264
44. analysis of positive transcription elongation factor - HEXIM1 - 7SK RNA complex PMID: 15994294
45. Role of P-TEFb as an activator of transcription elongation can be separated from its role in RNA processing; neither function is universally required for expression of mammalian pol II-dependent genes. PMID: 16308568
46. the interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct its binding to P-TEFb and subcellular localization that culminates in the inhibition of transcription PMID: 16362050
47. human cyclin T1 expression is sufficient to support Tat-mediated transactivation in primary mouse CD4 T lymphocytes and monocytes/macrophages and increases in vitro and in vivo HIV-1 production by these stimulated cells. PMID: 16439541
48. Using DNA microarray technology, we found that more than 20% of genes induced by PMA require cyclin T1 for their normal level of induction, and approximately 15% of genes repressed by PMA require cyclin T1 for their normal level of repression. PMID: 16764723
49. These data link the P-TEFb equilibrium to the intracellular transcriptional demand and proliferative/differentiated states of cells. PMID: 16980611
50. Prostratin induces Cyclin T1 expression and P-TEFb function and this is likely to be involved in prostratin reactivation of latent HIV-1 proviruses PMID: 17014716
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亞細胞定位：

Nucleus.
蛋白家族：

Cyclin family, Cyclin C subfamily
組織特異性：

Ubiquitously expressed.
數據庫鏈接：

HGNC: 1599

OMIM: 143055

KEGG: hsa:904

STRING: 9606.ENSP00000261900

UniGene: Hs.92308