Recombinant Rat Acetylcholinesterase (Ache)

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品名簡稱：

Recombinant Rat Ache protein
貨號：

CSB-EP001154RA
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Ache
Uniprot No.：

P37136
別名：

(AChE)
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

68.9 kDa
表達區域：

32-614aa
氨基酸序列

EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGILDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLIWIYGGGFYSGASSLDVYDGRFLAQVEGTVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELISCLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPDALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPAHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTVEERIFAQRLMQYWTNFARTGDPNDPRDSKSPRWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
基因功能參考文獻：
1. sepsis decreased AChE activity by reducing its expression at the neuromuscular junction. PMID: 28849127
2. Paraquat-induced death of hippocampal neurons was partially mediated by AChE variants inducing and cholinergic and gultamatergic transmission disruption. PMID: 28916328
3. Quercetin improves memory and anxiogenic-like behavior in diabetic rats by preventing the increase in Ache activity seen in streptozocin induced diabetes. PMID: 27694000
4. the induction of PRiMA-linked AChE in osteoblast should be independent to classical estrogen signaling pathway. PMID: 27019979
5. This study showed that changes in AChE may, at least in part, to lead to memory impairment caused by galactose. Taken together, our results can help understand the etiopathology of classical galactosemia. PMID: 26948151
6. Acetylcholinesterase activity was increased in rats subjected to mild hyperhomocysteinemia PMID: 24590316
7. Hindlimb unloading (simulation of hypogravity) leads to an increase in functional activity of acetylcholinesterase on the background of reduced postsynaptic membrane area occupied by acetylcholine receptors. PMID: 25711085
8. Under all studied experimental conditions, offspring brain acetylcholinesterase (AChE) activity was found to be significantly decreased due to maternal hypothyroidism PMID: 24632022
9. AChE has an important role in modulation of early immune responses against Toxoplasma gondii infection. PMID: 24039284
10. Spindle-shaped AChE-rich cells in the floccular stalk project to the flocculus, nodulus and uvula, but not to the cerebellar hemispheres. PMID: 24010172
11. Tyrosine increased acetylcholinesterase in blood & all tested brain areas. Acute administration decreased its mRNA levels in hippocampus. Chronic administration did the same in the hippocampus. PMID: 23046746
12. Acetylcholinesterase inhibitor huperazine A improved, or partly reversed, the Abeta-induced damage of neurite outgrowth. PMID: 23119107
13. in the absence of pituitary growth hormone the postnatal proliferation of cholinergic synapses in the rat spinal cord where AChE activity is abundant, is markedly reduced PMID: 22922167
14. Hypothyroidism significantly reduced both specific and total AChE activity in spinal cord of rat pups PMID: 22982053
15. The results from the present study demonstrate a marked increase in AChE activity in all brain structures following the administration of a branched-chain amino acid pool. PMID: 22328136
16. This study demonistrated that AChE positive neurons were stained lightly, then during later developmental periods the staining gradually increased and achieved adult level of intensity on the day P21 in spinal cord. PMID: 22028090
17. During induction of the non-alcoholic fatty liver disease with CCl4 AChE activity was significantly increased compared to control in blood, liver and brain tissues. PMID: 21881966
18. AChE activity can act as purinergic and cholinergic markers in lymphocytes PMID: 20832780
19. effect of melatonin on neuroinflammation and acetylcholinesterase activity induced by LPS in rat brain PMID: 20450904
20. These findings suggest that the restricted localization of PRiMA-linked G4 AChE at the nmjs could be contributed by the pre-synaptic motor neuron and/or the post-synaptic muscle fiber. PMID: 19680821
21. the importance of post-transcriptional mechanisms in regulating AChE expression in differentiating neurons and implicate HuD as a key trans-acting factor in these events PMID: 12468554
22. The activity of this protein is completely suppressed by the administration of LD50 of phosphacol. PMID: 12629810
23. AChE is necessary for normal dendrite and axon formation in hippocampal neurones and suggest that it may also play a role in excitatory synapse development, plasticity, and remodelling. PMID: 12799140
24. Local increment in neuronal AChE concentration in the mammalian hippocampus, such as in amyloid deposits, may play a role in triggering neuropathological and behavioral changes such as those observed in Alzheimer disease brains PMID: 12969266
25. In this study we show an effect of AChE that could contribute to the increased deposition of Amyloid beta PMID: 14678761
26. AChE is involved in regulating cell-matrix interactions in bone PMID: 15454088
27. Nearly all AChE-immunoreactive neurons in the Lateral hypothalamus express immunoreactivity for either melanin concentrating hormone or for orexin PMID: 15488307
28. rats subjected to ovariectomy presented a significant increase in brain Na+, K+-ATPase, AChE and CAT activities, but did not change the oxidative stress parameters PMID: 15869839
29. Results suggest that the presence of acetylcholinesterase protein doesn't correlate with catalytic activity, and the diffuse cytoplasmic and plasma membrane localization of AChE is a property of neuronal cell types. PMID: 16052514
30. action of P2Y1 receptor could lead Ache gene activation in cultured cortical neurons PMID: 16429571
31. Lead acetate decreased brain AChE activity of rats in dose/age dependent manner. PMID: 16678265
32. The SH-amino acids seem to affect the hippocampal enzyme indirectly, possibly by lipid(s)-protein modifications(s) and Hci by inducing oxidative stress, since no effect was observed on pure AChE activity PMID: 16773467
33. Results suggest that interferon-beta may act through inhibition of acetylcholinesterase, and ethidium bromide could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions. PMID: 16871442
34. In the cerebellum, hyperthyroidism provoked a significant decrease in both the AChE and the Na(+), K(+)-ATPase activities PMID: 17165152
35. findings provide evidence for intra- and supramodal influences of the neonatal removal of retinal input on neural activity- and use-dependent modifications of cortical AChE activity PMID: 17280650
36. In choline-deprived (CD) newborn rats,ANTIOXIDANT STATUS and AChE and Na(+),K(+)-ATPase activities were significantly reduced by 23%, 24% and 50%, respectively, in the brains of both sexes. PMID: 17484629
37. Low concentrations of aspartame metabolites had no effect on AChE activity, whereas high or toxic concentrations decreased enzyme activity. PMID: 17580119
38. that the 55-kDa AChE may be involved in different biological processes such as neural development, tumor progression, and angiogenesis. PMID: 17606622
39. These findings suggest that cell surface AChE, possibly in a novel signalling complex containing APP and perlecan, contributes to a generalised mechanism for polarised membrane protrusion and migration in all adherent cells. PMID: 17948252
40. Repeated exposure to low dose malathion increased brain AChE activity and altered antioxidant levels. PMID: 18341513
41. Data show that acetylcholine esterase enzyme activity shows a significant decrease in cerebral cortex and a increase in the muscle of experimental hypoxic neonatal rats. PMID: 18406032
42. Although the mechanisms of action of GAA on AChE activity and on memory are unclear, these findings suggest that the accumulation of GAA found in patients with GAMT-deficiency may be one of the mechanisms involved in neural dysfunction. PMID: 18437545
43. Tanshinone modulates AChE and NOS proteins concentrations in the hippocampus of AD rats. This may have therapeutic potential in AD rats. PMID: 18980714
44. Protein CutA undergoes an unusual transfer into the secretory pathway and affects the folding, oligomerization, and secretion of acetylcholinesterase. PMID: 19049969
45. cell death due to paraoxon not AChE dependent PMID: 19378919
46. Data show that after denervation, the expression of PRiMA, AChE(T) and G(4) AChE decreased markedly in the spinal cord, and in fast- and slow-twitch muscles. PMID: 19490106
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亞細胞定位：

Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein.; [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
蛋白家族：

Type-B carboxylesterase/lipase family
組織特異性：

Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver.
數據庫鏈接：

KEGG: rno:83817

UniGene: Rn.105879