ANPEP
ANPEP全稱為丙氨酰氨肽酶，又稱CD13或膜氨肽酶N，是一種廣泛分布于上皮細(xì)胞、免疫細(xì)胞及血管內(nèi)皮細(xì)胞表面的跨膜蛋白。ANPEP通過(guò)水解多肽鏈N末端的丙氨酸殘基參與調(diào)控細(xì)胞信號(hào)傳導(dǎo)，在腫瘤微環(huán)境中可促進(jìn)新生血管形成并介導(dǎo)免疫逃逸，其異常表達(dá)與多種惡性腫瘤密切相關(guān)，如急性髓系白血病、肝癌及胃癌的轉(zhuǎn)移進(jìn)程均觀察到CD13活性升高。ANPEP在類風(fēng)濕性關(guān)節(jié)炎、炎癥性腸病等免疫性疾病中也被發(fā)現(xiàn)參與炎癥細(xì)胞浸潤(rùn)過(guò)程。針對(duì)CD13的靶向藥物開(kāi)發(fā)已有突破，烏苯美司作為氨肽酶抑制劑已用于臨床輔助抗癌治療，而新型小分子抑制劑Tosedostat在復(fù)發(fā)型血液腫瘤的II期試驗(yàn)中展現(xiàn)出潛力。近年來(lái)CAR-T療法中CD13也被探索為實(shí)體瘤治療靶點(diǎn)，但其在正常組織的廣泛表達(dá)仍是藥物研發(fā)需要攻克的主要障礙。
熱銷產(chǎn)品
Recombinant Human Aminopeptidase N (ANPEP), partial (CSB-EP001827HU1)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ANPEP Recombinant Monoclonal Antibody (CSB-RA001827MA1HU)
驗(yàn)證數(shù)據(jù)
Immunofluorescence staining of HEPG2 cell with CSB-RA001827MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of A549 cell with CSB-RA001827MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay Peak curve showing 293 cells surface stained with CSB-RA001827MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.
Human alanyl (membrane) aminopeptidase (ANPEP)ELISA kit (CSB-EL001827HU)
驗(yàn)證數(shù)據(jù)
貨號(hào)：CSB-EL001827HU
規(guī)格：96T/48T
靈敏度：7.81 pg/mL
檢測(cè)范圍：31.25 pg/mL-2000 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ANPEP Antibodies
ANPEP for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA001827GA01HU | ANPEP Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA006158 | ANPEP Antibody | Human | WB, ELISA |
| CSB-PA008966 | ANPEP Antibody | Human | WB, IHC, ELISA |
| CSB-PA969898 | ANPEP Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-PA001827LA01HU | ANPEP Antibody | Human, Mouse | ELISA, WB, IHC, IF |
| CSB-PA001827LB01HU | ANPEP Antibody, HRP conjugated | Human | ELISA |
| CSB-PA001827LC01HU | ANPEP Antibody, FITC conjugated | Human | |
| CSB-PA001827LD01HU | ANPEP Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA993633A0HU | ANPEP Recombinant Monoclonal Antibody | Human | ELISA, IHC |
| CSB-RA001827MA1HU | ANPEP Recombinant Monoclonal Antibody | Human | ELISA, IF, FC |
ANPEP Proteins
ANPEP Proteins for Felis catus (Cat) (Felis silvestris catus)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827CA CSB-EP001827CA CSB-BP001827CA CSB-MP001827CA CSB-EP001827CA-B |
Recombinant Cat Aminopeptidase N (ANPEP), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ANPEP Proteins for Canis familiaris (Dog) (Canis lupus familiaris)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827DO CSB-EP001827DO CSB-BP001827DO CSB-MP001827DO CSB-EP001827DO-B |
Recombinant Dog Aminopeptidase N (ANPEP) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ANPEP Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827BO CSB-EP001827BO CSB-BP001827BO CSB-MP001827BO CSB-EP001827BO-B |
Recombinant Bovine Aminopeptidase N (ANPEP), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ANPEP Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827MO CSB-EP001827MO CSB-BP001827MO CSB-MP001827MO CSB-EP001827MO-B |
Recombinant Mouse Aminopeptidase N (Anpep), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ANPEP Proteins for Sus scrofa (Pig)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827PI CSB-BP001827PI CSB-MP001827PI CSB-EP001827PI-B |
Recombinant Pig Aminopeptidase N (ANPEP), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP001827PI | Recombinant Pig Aminopeptidase N (ANPEP), partial | E.coli |
ANPEP Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827HU CSB-EP001827HU CSB-BP001827HU CSB-MP001827HU CSB-EP001827HU-B |
Recombinant Human Aminopeptidase N (ANPEP), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP001827HU1 | Recombinant Human Aminopeptidase N (ANPEP), partial | E.coli |
ANPEP Proteins for Oryctolagus cuniculus (Rabbit)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827RB CSB-EP001827RB CSB-BP001827RB CSB-MP001827RB CSB-EP001827RB-B |
Recombinant Rabbit Aminopeptidase N (ANPEP), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ANPEP Proteins for Gallus gallus (Chicken)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827CH CSB-EP001827CH CSB-BP001827CH CSB-MP001827CH CSB-EP001827CH-B |
Recombinant Chicken Aminopeptidase N (ANPEP), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ANPEP Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001827RA CSB-BP001827RA CSB-MP001827RA CSB-EP001827RA-B |
Recombinant Rat Aminopeptidase N (Anpep), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP001827RA | Recombinant Rat Aminopeptidase N (Anpep), partial | E.coli |
ANPEP ELISA Kit
ANPEP ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL001827HU | Human alanyl (membrane) aminopeptidase (ANPEP)ELISA kit | serum, plasma, tissue homogenates | 7.81 pg/mL |
ANPEP ELISA Kit for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL001827MO | Mouse Aminopeptidase N(ANPEP) ELISA kit | serum, plasma, tissue homogenates | 9.7 mU/mL |
